Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis.
نویسندگان
چکیده
Transposon Tn916 was transferred from Streptococcus faecalis to Mycoplasma hominis by a mating process resembling conjugation with a frequency of 10(-6) to 10(-7). Tn916 was inserted into the mycoplasmal chromosome in single and multiple copies.
منابع مشابه
An Excision-Competent and Exogenous Mosaic Transposon Harbors the tetM Gene in Multiple Mycoplasma hominis Lineages.
The presence of the tetracycline resistance determinant tetM has been reported for Mycoplasma hominis (1, 2) and association with Tn916 genes established (3). To identify the mobile genetic element harboring this gene, genome analysis of M. hominis Sprott (isolated from a nongonococcal urethritis patient before 1978 [4]) and targeted PCR/amplicon sequencing of M. hominis 1630 (1984, arthritis i...
متن کاملNatural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria.
The conjugative streptococcal transposon Tn916 was found to transfer naturally between a variety of gram-positive and gram-negative eubacteria. Enterococcus faecalis hosting the transposon could serve as a donor for Alcaligenes eutrophus, Citrobacter freundii, and Escherichia coli at frequencies of 10(-6) to 10(-8). No transfer was observed with several phototrophic species. Mating of an E. col...
متن کاملSpecific binding of integrase to the origin of transfer (oriT) of the conjugative transposon Tn916.
Purified integrase protein (Int) of the conjugative transposon Tn916 was shown, using nuclease protection experiments, to bind specifically to a site within the origin of conjugal transfer of the transposon, oriT. A sequence similar to the ends of the transposon that are bound by the C-terminal DNA-binding domain of Int was present in the protected region. However, Int binding to oriT required ...
متن کاملHomology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916.
In several tetracycline-resistant (Tetr) Clostridium difficile strains, homology with the Tn916 part of plasmid pAM120 DNA was observed. This 15-kilobase transposon, carrying a Tetr determinant, was originally found in Streptococcus (Enterococcus) faecalis. Hybridization experiments revealed that at least six of seven HincII fragments of Tn916, representing greater than 95% of its length, showe...
متن کاملConjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase.
Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related transposons involves excision of a free, nonreplicative, covalently closed circular intermediate that is substrate for integration. Excisive recombination requires two transposon-encoded proteins, Xis-Tn and Int-Tn, whereas...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 169 8 شماره
صفحات -
تاریخ انتشار 1987